Poly(ADP-Ribose) Polymerase by Alexei V. Tulin

Author:Alexei V. Tulin
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

9.Wash pellet three times with binding/washing buffer with 5 min of gentle rotation at RT. Label and store wash supernatants.

10.Elute bound protein by mixing pellet with 100 μl of 1× SDS protein loading buffer and heating for 5 min at 95 °C. Vortex mixture for 1 min. Gently centrifuge and remove supernatant for protein gel/Western blot .

11. Human PARP-1 is detected on Western blot with anti-PARP-1 (mouse monoclonal, 1:500, Serotec), IgG is detected with anti-rabbit horseradish peroxidase (1:3000, Jackson ImmunoResearch Laboratories), and Drosophila PARP and its various deletion isoforms are detected with anti-His-tagged antibody (Abcam, ab9108).

Download

Copyright Disclaimer:
This site does not store any files on its server. We only index and link to content provided by other sites. Please contact the content providers to delete copyright contents if any and email us, we'll remove relevant links or contents immediately.